- Small and large vessel formation assays
- Fast track small vessel formation assays with INM
- 3D cell culture with sceMatrix
✓ Human bio-matrix biocompatible with endothelial cell migration and proliferation
✓ Dynamic state of endothelial cells minimize the number of cells required for the tube formation
✓ Ability to interrogate a multi-step complex vessel-forming process
✓ Captures the two stages of microvessel and that of macrovessel formation
✓ Ability to examine regression of vessel formation without endothelial cell apoptosis
✓ Tested for various types of cells such as primary endothelial cells, fibroblasts, and mesenchymal stem cells
✓ The complete process, including tissue remodeling, is accessible for microscopic observation and does not require tissue disruption
TUBE FORMATION ASSAY
Tube formation assays typically use an extracellular matrix -obtained from Engelbreth-Holm-Swarm mouse sarcoma- that inhibits endothelial cell growth and causes apoptosis soon after the process has been initiated. Using the ANGIOstream kit with its unique human stem cell derived ECM coated plates, endothelial cells can migrate, proliferate, differentiate, form micro- and macrovessels stable for months. The stability of vessels allows research of pro- or anti-angiogenic tendency of various drugs or toxic substances.
Brightfield microscopy image showing HUVEC cells micro-vessel formation after 2 weeks of incubation in the pre-coated plate of ANGIOstream in vitro Angiogenesis kit, magnification 100x
Brightfield microscopy image of a macro-vessel composed of HUVEC cells during a tube formation assay in the ANGIOstream pre-coated plates. The large vessels, their branching and sprouting, are visible to the naked eye without requiring any staining procedure, magnification 100x
Fluorescence microscopy image of Green Fluorescent Protein (GFP) expressing Human Umbilical Vein Endothelial Cells (HUVEC) forming a suspended macrovessel using ANGIOstream in vitro vessel generation assay pre-coated plate, magnification 100x
Brightfield microscopy image of a large suspended macrovessel using ANGIOstream in vitro Angiogenesis pre-coated plate incubated with Human Umbilical Vein Endothelial Cells (HUVEC). Macrovessel image taken after several weeks of incubation during a tube generation assay, magnification 40x.