APPLICATIONS
- Small and large vessel formation assays
- Fast track small vessel formation assays with INM
- 3D cell culture with sceMatrix
KIT COMPONENTS
Kit Name/Components
Storage
KEY ADVANTAGES
✓ Human bio-matrix biocompatible with endothelial cell migration and proliferation
✓ Dynamic state of endothelial cells minimizes the number of cells required for the in vitro assay
✓ Ability to investigate various drugs and substances for induced regression
✓ Allows for testing the regression of micro-vessels and macro-vessels, including that of suspended macro-vessels
✓ The establishment of stable vessels and absence of apoptosis, allows the examination of chronic effects of substances that lead to regression
✓ Plate containing a thin layer of stem cell derived extracellular matrix (ECM) optimal for Human Umbilical Vein Endothelial Cells (HUVECs)
✓ The complete process is accessible for microscopic observation and does not require tissue disruption
VESSEL REGRESSION ASSAY
The in vivo process of blood vessel regression, Angio-regression, is an integral component of vasculature maturation and remodeling. ANGIOstream™ unique in vitro Angio-regression; assay utilizes a human stem cell derived extracellular matrix to grow stable micro- and macro-vessels, including suspended macro vessels. This offers the opportunity to investigate various drugs and substances for induced regression.
The use of a simple, reproducible in vitro angio-regression assay will allow for the efficient prescreening of a multitude of compounds, it offers also a simple system for exploring the mechanisms involved in blood vessel regression.
The Angio-regression Kit contains media and reagents that have been optimized for the culturing of Human Umbilical Vein Endothelial Cells (HUVECs) onto plate containing a thin layer of the small mobile stem cell-derived extracellular matrix (SMS-ECM-1). The precoated plate has been optimized for the formation of both endothelial cell-derived micro-vessels and suspended macro-vessels which can be easily monitored microscopically.
Brightfield microscopy image of Human Umbilical Cord Vein Endothelial Cells (HUVEC) exhibiting obvious gross shape changes conducive to vessel formation, such as flattening and forming different sizes of vacuoles (including large vacuoles) within days. Magnification 100x
Fluorescence microscopy image of Green Fluorescent Protein (GFP) expressing Human Umbilical Vein Endothelial Cells (HUVEC) forming a suspended macrovessel using ANGIOstream in vitro vessel generation assay pre-coated plate, magnification 100x
