APPLICATIONS
- Fast track small vessel formation assay with INM
- Small and large 3D vessel formation assay
- 3D cell culture with sceMatrix
KEY ADVANTAGES
✓ INM induces fast differentiation and reorganization of endothelial cells, resulting in microvessel formation
✓ The directed process of polygonal microvessel organization involves cell communication, relocation, spatial collective organization, differentiation and is visible within 2-3 hours
✓ Fast induction of INM minimizes the time required for tube formation of endothelial cells
✓ Easy handling with sterile, thin-head forceps
✓ Several INMs can be applied per cell culture well
✓ The complete process, including tissue remodeling, is accessible for microscopic observation and does not require tissue disruption
ANGIOGENESIS MICROVESSEL FORMATION ASSAY
Angiogenesis microvessel formation assays typically use an extracellular matrix -obtained from Engelbreth-Holm-Swarm mouse sarcoma cells. This extracellular matrix inhibits endothelial cell migration and proliferation and causes apoptosis (cell death) soon after the capillary like structures have been formed (within hours). INM feature is a faster approach for microvessel formation when used with the ANGIOstream kit. Microvessel formation involves cell communication, migration, spatial collective organization, differentiateion that lead to a polygonal tissue organization. The process starts to be visible within 2-3 hours and is restricted to the area covered by INM.
Brightfield microscopy image showing INM covered side (left) where endothelial cell differentiation and organization occurs after 2 hours of incubation, in contrast to absence of changes outside INM. Cells were cultured using ANGIOstream coated plates. Magnification 40x
Brightfield microscopy image of microvessels composed of HUVECs during a tube formation assay using INM and the ANGIOstream kit. The polygonal organization of small vessels are reminiscent of capillary networks in vivo. Magnification 40x
Brightfield microscopy image of microvessels composed of HUVECs during a tube formation assay using INM and the ANGIOstream kit. Endothelial cells are exhibiting gross structural changes and are interconnected forming a polygonal organization of small vessels reminiscent of capillary networks in vivo. Magnification 100x
