✓ The complex reorganization starts after 2D cell confluence is achieved
✓ Ability to shift from two-dimensional (2D) cell culture to three-dimensional (3D) cell culture environment
✓ No need for any scaffolding or special cell culture surfaces
✓ MSC aggregates and spheroids are shown to exhibit better self-renewal differentiation activities and up-regulation of paracrine secretion
✓ Potential for enhancement of the therapeutic efficacy of mesenchymal stem cells (MSCs)
✓ Scaffold-free platform allows cells to actively reorganize into a complex 3D cell assembly in the shape of spheroids
✓ The complex assembly of 3D MSC spheroids are stable for many days
UMBILICAL CORD MESENCHYMAL STEM CELL ASSEMBLY
The Intrinsic properties of many types of cells are critically altered when cell culture condition shifts from two-dimensional (2D) to three-dimensional (3D) environment. Currently, several lines of evidence have demonstrated the therapeutic potential of mesenchymal stem cells (MSCs) in regenerative medicine.
The advantages of 3D grown MSC include the promotion of self-renewal and differentiation activities as well as up-regulation of paracrine secretion. Following the development of 3D cell culture, the enhanced therapeutic efficacy of spheroid-forming MSCs have been identified in several animal disease models by promoting differentiation or trophic factor secretion, as compared to planar-cultured MSCs.
Spheroids are produced using different techniques such as hanging drop or low attachment surfaces. These techniques rely on the cells attaching to each other by the lack of another alternative. So, in this scaffold-free platform, cells are organized into the cell spheroids and floated in the culture medium due to the lack of tangible supporting materials.
In contrast to that, the medium stemInduce ™ MAG from SMSbiotech induces the active reorganization of umbilical cord mesenchymal stem cells that grown on a regular cell adherent plate. The cells migrate towards a center and organize into a complex 3D cell assembly. The initiation of this complex organization follows immediately after 2D cell confluency and continues for many days.
Umbilical Cord Derived Mesenchymal Stem Cell (MSC) Inductive Assembly using the medium stemInduce MAG. Magnification 40x
Umbilical cord derived MSC inductive co-assembly with GFP expressing endothelial cells using the medium stemInduce™ MAG. The drawn circle indicates the assembly`s borders. Magnification 100x